If the PROVEAN score is equal to or below a predefined threshold (e.g. The scores are then averaged within and across clusters to generate the final PROVEAN score. The top 30 clusters of closely related sequences form the supporting sequence set, which will be used to generate the prediction.Ī delta alignment score is computed for each supporting sequence. Briefly, clustering of BLAST hits is performed by CD-HIT with a parameter of 75% global sequence identity. Single or multiple amino acid deletionsĪn overview of the PROVEAN procedure is shown in Figure 1.Single or multiple amino acid insertions.Single or multiple amino acid substitutions.PROVEAN is able to provide predictions for any type of protein sequence variations including the following. PROVEAN was developed to predict whether a protein sequence variation affects protein function. How closely matching sets of genes can belong to entirely different species is one of the great mysteries of modern biology.The results below are based on PROVEAN v1.1. However, not all differences between species can be explained by gene differences alone. Because of this close similarity, scientists think that it is the sequence of genes, as well as the types of genes themselves, that account for most of the differences between the two species.

Just how different are the genes making up different life forms? In the case of the human and the chimp, not much: about 98 percent of the DNA in a chimpanzee cell is identical to the DNA in a human cell.

The kinds of proteins, the amounts, and the order in which they are made all help determine how one type of cell differs from another and, ultimately, how one species of organism differs from another. These proteins are used to repair cells and make new ones. Genes contain the chemical information needed to create different kinds of proteins. Each gene is a specific segment of DNA, occupying a fixed place on a chromosome. Did You Know? What makes a human different from a chimpanzee? Much of the answer lies in the genes, the basic units of heredity.

Gene sequence definition